ABSTRACT Passiflora cincinnata Mast. is a wild passion fruit species widely distributed in Brazil, with diverse economic potential and source of resistance to biotic and abiotic stress factors. Despite this, molecular genetic studies on this species are incipient, which limits the definition of priority regions for conservation and identification of accessions to be prospected for germplasm banks and inserted in genetic improvement programs. Thus, this study aimed to characterize the structure and genetic diversity of P. cincinnata accessions using inter simple sequence repeat (ISSR) markers. The study was carried out using leaf samples from 213 accessions of P. cincinnata, with 158 accessions from six natural populations (collectively considered in this study as a hypothetical metapopulation) in the state of Bahia, Brazil, and the other 55 accessions from two collections of Embrapa Cerrados and Embrapa Cassava & Fruits active germplasm banks (AGBs). Genetic estimates were based on an analysis of the amplification profile of 12 ISSR primers. Descriptive statistics analysis and the polymorphic information content (PIC) and expected heterozygosity (He) estimates were carried out using the GENES software. Approaches based on the Bayesian model, principal coordinate analysis (PCoA), and analysis of molecular variance (AMOVA) were used to assess the genetic structure. Passiflora cincinnata populations/collections showed high polymorphism rates. The metapopulation showed 25.3% of private markers/alleles. The metapopulation is structured in at least three genetic groups, while AGBs in two genetic groups. AMOVA indicated higher diversity within populations, with low differentiation between them.

Lippia rotundifolia is an aromatic species, native and endemic to rocky fields, which are isolated by small mountains. It is little known about their chemical composition. Because of that we aimed to study the chemical diversity of volatiles released from rosemary leaves (Lippia rotundifolia Cham.) coming from 11 populations of the Minas Gerais, Brazil. The material for chemical analysis was obtained from the accessions in the active germplasm bank of the Instituto de Ciências agrárias of the Universidade Federal de Minas Gerais. A total of 67 compounds were detected by HS-CG-MS. Most volatiles were detected in the São Gonçalo do Rio das Pedras accession, with 40 compounds, whereas in the Santana do Riacho accession only 5 compounds were detected. Volatiles myrcene, linalool and caryophyllene were detected in the majority of the accessions. We concluded that Lippia rotundifolia presents high chemical variability. The volatiles mircene, linalool and caryophyllene are the most frequent, detected in most of the accessions. Due to some accession to present predominance of monoterpenes, others sesquiterpenes, studies of molecular analysis are necessary for associate the chemotypes, since environmental variation influence the gene expression of the secondary metabolites.

Objetivou-se avaliar o potencial físico e fisiológico de sementes de barbatimão oriundas de três locais (acessions). As se-mentes foram retiradas das vagens, quantificadas e separadas em viáveis e danificadas. Após escarificação mecânica de algumas sementes, foi comparada a germinação destas com a das não escarificadas. O potencial físico das sementes de barbatimão foi determinado a partir da quantidade de sementes por fruto, sementes danificadas, peso de 100 sementes, teor de água e medidas biométricas.. O potencial fisiológico foi avaliado pelo teste de germinação, primeira contagem do teste de germinação, índice de velocidade de germinação e comprimento de plântulas. Pelos resultados foi possível verificar reduzido percentual de sementes danificadas. As menores sementes apresentaram-se mais viáveis sem danificação física no tegumento. A largura das sementes não diferiu estatisticamente nos três acessions, assim como a massa fresca da raiz foi beneficiada pelo tegumento intacto, não diferindo entre os acessions. Contudo o tegumento escarificado apresentou-se superior ao tegumento intacto com apenas 5,10% de sementes inviáveis afetadas por fungos. Para melhores resultados sugere-se a realização de novos estudos com diferentes épocas de armazenamento das sementes dos mesmos acessions.

The aim this study was to evaluate the potential physical and physiologic of seeds of barbatimão from three origins. The seeds were removed from pods, quantified and separated in to viable and damaged. The seeds were mechanically scarified and had germination compared to intact seeds. The physical potential of seeds of barbatimão was determined from the quantity of seeds, damaged seeds, weight of 100 united of seeds, water content and biometric measurements. the physiological potential was evaluated by germination test, count firs of germination test, speed rate of germination and seedling length. The results showedthat there was a low percentage of damaged seeds and the smaller seeds showed itselves more viable without physical damage to the tegument. The width of the seeds was similar in all three accesses as well as the fresh root that was benefitted by intact tegument and did not differ between accesses. However the scarified tegument showed up to the top intact tegument with only 5.10% of viable seeds affected by fungus. For better results it is suggested further studies with different times of seed storage of the same access.